Fig. 2: CircRPPH1 was enriched in BC and knockdown of circRPPH1 inhibited the growth of BC in vitro.

a The expression of circRPPH1 was detected in BC tissues and their paired adjacent tissues (N = 40). b The expression of circRPPH1 in BC cells (MDA-MB-231, MCF-7, and BT549) was compared to that in mammary epithelial cells, MCF-10A. c CircRPPH1 levels were detected after transfecting BC cells with si-NC or si-circRPPH1. d–e Effect of si-circRPPH1 on the proliferation of MDA-MB-231 (d) and MCF-7 (e) cells were investigated by MTT assay. f–g The migration of MDA-MB-231 cells was analyzed by wound-healing assay. Photographs were taken at 0 h and 24 h after scratching (f). Wound closure was analyzed (g). h–i Transwell assay was conducted to analyze the migration of MDA-MB-231 cells. Photographs were taken at 20 h after seeding (h). Cell numbers were counted (i). j–k Effect of si-circRPPH1 on colony formation ability of BC cells (j). The number of cell colonies were counted (k). l–m The levels of PCNA protein were analyzed by western blotting. ACTIN was employed as internal control. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.