Fig. 3: CLDN4 is a downstream target of SPTBN2 in UCEC.
From: SPTBN2 regulated by miR-424-5p promotes endometrial cancer progression via CLDN4/PI3K/AKT axis
a The top ten genes with the best positive correlation were revealed through co-expression analysis with SPTBN2 in the TCGA UCEC database. b A positive correlation between SPTBN2 and CLDN4 was found in the TCGA database and starBase-database. c KEGG analysis indicated that CLDN4 expression was closely correlated with the expression of PI3K/AKT pathway gene sets by WEB-based Gene Set Analysis Toolkit (http://www.webgestalt.org/). d The protein expression of CLDN4 and the genes related to PI3K/AKT pathway were examined in UCEC cells after SPTBN2 knockdown. e Proteins extracted from UCEC cells with SPTBN2 knockdown were incubated with antibody for SPTBN2 and subjected to IP/Western blot to investigate the interaction. f The mRNA expression of CLDN4 in UCEC and normal tissues from TCGA datasets. g, h Expression of CLDN4 in UCEC based on patient’s age and individual cancer stages from TCGA datasets. i The mRNA expression of CLDN4 in 24 paired UCEC tissues and normal tissues. j, l Representative IHC images of CLDN4 expression in UCEC and normal tissues(l), IHC score for CLDN4 in the clinical tissues (j). k Correlation between SPTBN2 and CLDN4 in UCEC tissues. m Kaplan–Meier analysis of UCEC cohorts based on predictive survival analysis. (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. The data expressed as the mean ± SD).
