Fig. 6: SPTBN2 is a direct target of miR-424-5P.

a, b Screening database speculated that the 3'-UTR binding site of SPTBN2 might bind to the related miRNA. c SPTBN2 expression after transfected with miR-195-5p mimics, miR-497-5p mimics, and miR-424-5p mimics in Ishikawa cell were detected by qRT-PCR. d The expression of miR-424-5p in 24 paired UCEC tissues and normal tissues. e The correlation between SPTBN2 and miR-424-5p in 24 UCEC tissues. f The mRNA and protein expression of SPTBN2 in Ishikawa cells was determined by qRT-PCR and Western blot after transfected with miR-424-5p mimics, miR-424-5p mimics negative control (miR-NC), miR-424-5p inhibitor, and miR-424-5p inhibitor negative control (miR-in-NC). g Wild type (WT) and Mutant type (MUT) SPTBN2 3' UTR sequences were cloned into pmirGLO luciferase reporter vector. h 293 T cells were co-transfected with miR-424-5p mimics, negative control, and luciferase reporters containing the wild type or mutated transcripts of SPTBN2 3′UTR as indicated. (*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. The data expressed as the mean ± SD).