Fig. 7: MiR-124 targeted and inhibited COL4A1. | Cell Death Discovery

Fig. 7: MiR-124 targeted and inhibited COL4A1.

From: LncRNA SND1-IT1 facilitates TGF-β1-induced epithelial-to-mesenchymal transition via miR-124/COL4A1 axis in gastric cancer

Fig. 7

A The expression level of COL4A1 was determined in tumor tissues (n = 52) by RT-qPCR and compared to adjacent non-tumor tissues (n = 52). B Spearman correlation analysis between miR-124 and COL4A1 in GC tissues. C The expression level of COL4A1 was determined in four selected GC cells and GES1 cells by RT-qPCR and immunoblots. D Putative miR-124 binding sites in the COL4A1 mRNA 3′UTR in the starbase database. E Luciferase activity of the promoter of the reporter gene containing COL4A1-wt or COL4A1-mut in the presence of miR-124 mimic. F Representative immunoblots and quantitative analysis of COL4A1 protein in response to exogenous addition of miR-124 mimic and miR-124 inhibitor. Values are expressed as mean ± SD from three technical repeats. **P < 0.01, ***P < 0.001.

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