Fig. 8: MiR-124 attenuated TGF-β1-stimulated EMT in GC by targeting COL4A1. | Cell Death Discovery

Fig. 8: MiR-124 attenuated TGF-β1-stimulated EMT in GC by targeting COL4A1.

From: LncRNA SND1-IT1 facilitates TGF-β1-induced epithelial-to-mesenchymal transition via miR-124/COL4A1 axis in gastric cancer

Fig. 8

A The expression level of COL4A1 was determined by RT-qPCR in TGF-β1-stimulated HGC-27 cells with miR-124 mimic and COL4A1 expression vector. B CCK-8 assays were performed to examine the viability in TGF-β1-stimulated HGC-27 cells with miR-124 mimic and COL4A1 expression vector. C Representative photomicrographs of TGF-β1-stimulated HGC-27 cells migrating or invading from upper transwell chambers into bottom ones upon treatment with miR-124 mimic and COL4A1 expression vector. D Quantitative analysis of TGF-β1-stimulated HGC-27 cells migrating or invading from upper transwell chambers into bottom ones upon treatment with miR-124 mimic and COL4A1 expression vector. E Representative immunoblots and quantitative analysis of E-cadherin and N-cadherin in TGF-β1-stimulated HGC-27 cells treated with miR-124 mimic and COL4A1 expression vector. The results were normalized to the β-actin expression. Values are expressed as mean ± SD from three technical repeats. *P < 0.05, **P < 0.01, ***P < 0.001.

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