Fig. 4: Silencing EGR1 inhibits HCC progression.

A, B EGR1 expression in HCC cells tested by RT-qPCR and western blot analysis after upregulating or downregulating EGR1; C OD value tested by MTT test after upregulating or downregulating EGR1; D Colony-forming ability of HCC cells tested by colony formation assay after upregulating or downregulating EGR1; E Migration and invasion of HCC cells tested by Transwell test after upregulating or downregulating EGR1 (scale bar = 50 μm); F Migration distance of HCC cells tested by scratch test after upregulating or downregulating EGR1 (scale bar = 200 μm); G Apoptosis of HCC cells tested by flow cytometry after upregulating or downregulating EGR1. N = 3. The data were expressed as mean±standard deviation and compared by one-way ANOVA. &p < 0.05 vs. the oe-NC group; ^p < 0.05 vs. the si-NC group.