Fig. 4: Mettl14 downregulated EEF1A2 in SCI by mediating m6A methylation of EEF1A2. | Cell Death Discovery

Fig. 4: Mettl14 downregulated EEF1A2 in SCI by mediating m6A methylation of EEF1A2.

From: METTL14 promotes apoptosis of spinal cord neurons by inducing EEF1A2 m6A methylation in spinal cord injury

Fig. 4

A possible m6A methylation sites of EEF1A2; (B) m6A level in spinal cord tissues and H/R neurons detected by Dot blot assay; (C) expression of Mettl14 in rat spinal cord tissues detected by Western blot (N = 5); (D) expression of Mettl14 in spinal cord tissues detected by immunofluorescent staining (N = 5); (E) expression of Mettl14 in H/R neurons detected by Western blot; (F): expression of Mettl14 detected by Western blot; (G) total m6A level detected by Dot blot assay; (H) mRNA expression of EEF1A2 detected by RT-qPCR; (I) EEF1A2 protein expression detected by Western blot; (J) m6A modification level of EEF1A2 detected by Me-RIP assay. Data were expressed as mean ± SD and analyzed using independent t test. The experiment was repeated three times. * vs sham group, control group, sh-NC group, p < 0.05; # vs oe-NC group, p < 0.05.

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