Fig. 6: CAPN1 siRNA ameliorated burn plasma-induced cardiac cell injury. | Cell Death Discovery

Fig. 6: CAPN1 siRNA ameliorated burn plasma-induced cardiac cell injury.

From: Impairment of μ-calpain activation by rhTNFR:Fc reduces severe burn-induced membrane disruption in the heart

Fig. 6

A Representative blots and densitometric analysis showing siRNA-mediated knockdown of CAPN1 and the proteolysis of α-spectrin. B Blockade of CAPN1 with siRNA improved cell viability and reduced caspase-3 activity and PARP-1 degradation. GAPDH served as a loading control. Each independent data with the mean is presented. n = 6. Western blotting was performed in six independent biological experiments, and there were three technical replicates per sample. ##, **P < 0.01.

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