Fig. 7: RhTNFR:Fc mitigated calpain activation and myocardial damage caused by burns. | Cell Death Discovery

Fig. 7: RhTNFR:Fc mitigated calpain activation and myocardial damage caused by burns.

From: Impairment of μ-calpain activation by rhTNFR:Fc reduces severe burn-induced membrane disruption in the heart

Fig. 7

A Representative blots and the results showing membrane recruitment of calpain 1 (CAPN1) and the fragments of α-spectrin. NHE and GAPDH were used as the loading controls for membrane proteins and total proteins, respectively. B Plasma levels of LDH and cTnI. C The results of caspase-3 activity and representative blots and densitometric analysis of PARP-1 with GAPDH as an internal control. D The results showing heart function and mean arterial pressure (MAP). Each independent data with the mean is presented. n = 6 rats in each group. Western blotting was performed in six independent biological experiments, and there were three technical replicates per sample. *P < 0.05, **P < 0.01 vs. the burn group. M + Burn refers to rats that received rhTNFR:Fc 30 min post-burn.

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