Fig. 2: Cell death induced by BH3 mimetics in 2D and 3D cultures. | Cell Death Discovery

Fig. 2: Cell death induced by BH3 mimetics in 2D and 3D cultures.

From: Pediatric multicellular tumor spheroid models illustrate a therapeutic potential by combining BH3 mimetics with Natural Killer (NK) cell-based immunotherapy

Fig. 2

A RH30-GFP, RD-GFP and Kym-1 cells were cultured as adherent monolayer cells (2D) before treatment with different concentrations of BH3 mimetics for 48 h. Viability was analyzed using Cell Titer Glo® assay. B RH30-GFP, RD-GFP and Kym-1 cells were cultured as spheroids (3D) for 3 days before treatment with different concentrations of BH3 mimetics for 48 h. Viability was analyzed using CellTiter-Glo® assay. Data shown are mean + S.D. (n = 3). Synergy was quantified using Synergyfinder and expressed as Bliss Score with a score >0 indicating synergy on a scale to 100.

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