Fig. 3: Microscopic analysis confirms cell death induced by BH3 mimetics. | Cell Death Discovery

Fig. 3: Microscopic analysis confirms cell death induced by BH3 mimetics.

From: Pediatric multicellular tumor spheroid models illustrate a therapeutic potential by combining BH3 mimetics with Natural Killer (NK) cell-based immunotherapy

Fig. 3: Microscopic analysis confirms cell death induced by BH3 mimetics.The alternative text for this image may have been generated using AI.

A mRNA expression levels of BCL2 family members were compared in 3D spheroids and 2D culture of RH30-GFP and RD-GFP cells by qRT-PCR. Data shown are mean + SEM (n = 4). B–E RH30-GFP or RD-GFP cells were cultured as 3D spheroids for 3 days before exposure to A1331852 (0.25 μM RD-GFP, 0.1 μM RH30-GFP) or S63845 (0.3 μM RD-GFP, 0.03 μM RH30-GFP) either alone or in combination for 48 h. Cell death was assessed by PI staining for 30 min before analysis. B, C PI and GFP fluorescence was quantified and expressed as the ratio of PI/GFP. Data shown are mean + S.D. (n = 3), **p < 0.01; ***p < 0.001. D–E Exemplary images are shown for PI and GFP fluorescence. Scale bar equals 500 μm.

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