Fig. 4: Morphological assessment of spheroids. | Cell Death Discovery

Fig. 4: Morphological assessment of spheroids.

From: Pediatric multicellular tumor spheroid models illustrate a therapeutic potential by combining BH3 mimetics with Natural Killer (NK) cell-based immunotherapy

Fig. 4: Morphological assessment of spheroids.The alternative text for this image may have been generated using AI.

A, B RD-GFP cells cultured as 3D spheroids were left untreated (A) or exposed to the combination of A1331852 (0.25 μM) and S63845 (0.3 μM) (B) for 48 h before PI staining and light-sheet microscopy. C, D RD-GFP cells were cultured as large spheroids containing 50,000 cells for 7 days and either left untreated (C) or treated with the combination of A1331852 (0.25 μM) and S63845 (0.3 μM) (D) for 6 h before formalin fixation and H/E staining (lower right side) or analysis of Ki-67 staining by immunohistochemistry (large image and upper right side).

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