Fig. 7: Caspase dependency of BH3 mimetic sensitization towards NK cell killing. | Cell Death Discovery

Fig. 7: Caspase dependency of BH3 mimetic sensitization towards NK cell killing.

From: Pediatric multicellular tumor spheroid models illustrate a therapeutic potential by combining BH3 mimetics with Natural Killer (NK) cell-based immunotherapy

Fig. 7: Caspase dependency of BH3 mimetic sensitization towards NK cell killing.The alternative text for this image may have been generated using AI.

A RH30 and RD wild-type spheroids were pretreated with either S63845 (0.3 μM) or A1331852 (0.3 μM) for 4 h, before addition of NK cells. After 24 h of NK cell co-cultivation, caspase activity was stained using a CellEvent™ Caspase 3/7 green detection substrate. TL: transmitted light channel. B To inhibit caspase activity or TRAIL, GFP expressing RMS spheroids were co-cultured with zVAD.fmk (50 μM) or anti-TRAIL blocking antibody (1 μg/ml) in combination with BH3 mimetics for 4 h before addition of NK cells (E:T ratio of 1:1). To visualize cell death, spheroids were counterstained with PI after 72 h of NK cell co-culture. Data shown are mean + S.D. (n = 3). Scale bar equals to 500 μm.

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