Fig. 6: ZC3H15 inhibited the transcription of FBXW7 in GC cells.
From: ZC3H15 promotes gastric cancer progression by targeting the FBXW7/c-Myc pathway
A Western blot assays were used to examine the protein expression of indicated cells. B Dual-luciferase reporter assays were used to detect the promoter activity of FBXW7. C ChIP assay was performed by using Flag antibody. IgG was used as the negative control. D, E Western blot assays were performed to detect FBXW7 and c-Myc protein expression of indicated cells. F, G MTT assays were performed to detect the effects of FBXW7 downregulation on the proliferation of ZC3H15-knockdown HGC-27 and MKN-45 cells. H, I Transwell assays were performed to detect the effects of FBXW7 downregulation on the cell migration and invasion of ZC3H15-knockdown HGC-27 and MKN-45 cells. All data were expressed as mean ± SD. Student’s t-test was performed to analyzed significance, *P < 0.05, **P < 0.01, ***P < 0.001.
