Fig. 4: m6A enrichment is negatively associated with TRERNA1 activity in DLBCL. | Cell Death Discovery

Fig. 4: m6A enrichment is negatively associated with TRERNA1 activity in DLBCL.

From: ALKBH5-mediated N6-methyladenosine modification of TRERNA1 promotes DLBCL proliferation via p21 downregulation

Fig. 4

A Schematic representation of the position of m6A motifs within TRERNA1. B Changes in m6A-modified TRERNA1 levels between wild-type and mutants in SU-DHL4 cells; A-G mutant, mutant with A-G transition mutations. C TRERNA1 expression in control and shTRERNA1 SU-DHL4 cells in the presence of the indicated overexpression constructs. D Cell viability in the shControl- and shTRERNA1-treated SU-DHL4 cells in the presence of the indicated overexpression constructs. E Relative luciferase activity of TRERNA1 with either wild-type or A–G mutant m6A sites after the transfection of OCI-Ly3 cells with LV-ALKBH5. F Relative luciferase activity of TRERNA1 with either wild-type or A–G mutant m6A sites after the transfection of SU-DHL4 cells with LV-shALKBH5. G, H TRERNA1 levels in siALKBH5 and control cells were quantified by qRT-PCR at the indicated time points after actinomycin D treatment. The cells were treated with 5 μg/ml of actinomycin D at 0, 2, 4, 6, and 8 h. The data are presented as the mean ± SD; n = 3. *P < 0.05, **P < 0.01. ns nonsignificant.

Back to article page