Fig. 2: miR-181a–2–3p could be carried to SH-SY5Y cells through MSC–EVs. | Cell Death Discovery

Fig. 2: miR-181a–2–3p could be carried to SH-SY5Y cells through MSC–EVs.

From: MicroRNA-181a–2–3p shuttled by mesenchymal stem cell-secreted extracellular vesicles inhibits oxidative stress in Parkinson’s disease by inhibiting EGR1 and NOX4

Fig. 2: miR-181a–2–3p could be carried to SH-SY5Y cells through MSC–EVs.

A Abundance of miR-181a–2–3p in MSCs and MSC-derived EVs. B miR-181a–2–3p expression by RT-qPCR. C EVs observed under the TEM. D EV diameter detected using dynamic light scattering. E EV surface markers detected using Western blot. F Uptake of MSC–EV by SH-SY5Y cells detected using EV PKH67 tracing method. G Cy3-labeled miR-181a–2–3p entered SH-SY5Y cells via MSC–EV (200×). H miR-181a–2–3p expression in the SH-SY5Y cells. *p < 0.05 vs. SH-SY5Y blank, ***p < 0.001, NS insignificant difference. Cell experiments were repeated three times independently.

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