Fig. 6: EGR1 promotes apoptosis and OS of SH-SY5Y cells by regulating NOX4. | Cell Death Discovery

Fig. 6: EGR1 promotes apoptosis and OS of SH-SY5Y cells by regulating NOX4.

From: MicroRNA-181a–2–3p shuttled by mesenchymal stem cell-secreted extracellular vesicles inhibits oxidative stress in Parkinson’s disease by inhibiting EGR1 and NOX4

Fig. 6: EGR1 promotes apoptosis and OS of SH-SY5Y cells by regulating NOX4.

A Significant co-expression of EGR1 and NOX4 analyzed using MEM. B EGR1 enriched in the NOX4 promoter region verified by ChIP assay. C NOX4 mRNA level in SH-SY5Y cells treated with 6-OHDA at different concentrations detected using RT-qPCR. D NOX4 protein level in SH-SY5Y cells treated with 6-OHDA at different concentrations. E The knockdown effect of the two interference sequences of NOX4. F Expression of EGR1 and NOX4 in SH-SY5Y cells after different treatments measured using RT-qPCR. G Cell proliferation in SH-SY5Y cells after different treatments. H Cell apoptosis in SH-SY5Y cells after different treatments. I SOD level in SH-SY5Y cells after different treatments. J MDA level in SH-SY5Y cells after different treatments. K ROS levels in SH-SY5Y cells after different treatments detected using DCFH-DA fluorescent staining. *p < 0.05 vs. si-NC, oe-NC + si-NC, or SH-SY5Y cells without treatment, #p < 0.05 vs. oe-NC + si-NOX4, oe-NC + si-NC; $p < 0.05 vs. oe-NC + si-NOX4. Cell experiments were repeated three times independently.

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