Fig. 8: miR-181a–2–3p inhibits apoptosis and OS of SH-SY5Y cells by regulating the EGR1/NOX4 axis. | Cell Death Discovery

Fig. 8: miR-181a–2–3p inhibits apoptosis and OS of SH-SY5Y cells by regulating the EGR1/NOX4 axis.

From: MicroRNA-181a–2–3p shuttled by mesenchymal stem cell-secreted extracellular vesicles inhibits oxidative stress in Parkinson’s disease by inhibiting EGR1 and NOX4

Fig. 8

A miR-181a–2–3p, EGR1, and NOX4 expression in SH-SY5Y cells treated with 6-OHDA in combination with different plasmids determined using RT-qPCR. B Levels of p–p38 and p38 in SH-SY5Y cells treated with 6-OHDA in combination with different plasmids determined using Western blot analysis. C Cell proliferation in SH-SY5Y cells treated with 6-OHDA in combination with different plasmids. D Cell apoptosis in SH-SY5Y cells treated with 6-OHDA in combination with different plasmids. E SOD level in SH-SY5Y cells treated with 6-OHDA in combination with different plasmids detected using SOD kit. F MDA level in SH-SY5Y cells treated with 6-OHDA in combination with different plasmids detected using MDA kit. G ROS levels in SH-SY5Y cells treated with 6-OHDA in combination with different plasmids detected using DCFH-DA fluorescent staining. C, E, F, and G, *p < 0.05 vs. NC mimic + oe-NC or SH-SY5Y cells without treatment, #p < 0.05 vs. miR-181a–2–3p mimic + oe-NC or NC mimic + oe-NC; $p < 0.05 vs. miR-181a–2–3p mimic + oe-NC. Cell experiments were repeated three times independently.

Back to article page