Fig. 3: miR-344b-5p was down-regulated and mediates IH-induced cells injury.

A The expression of miR-344b-5p was measured by qRT-PCR after IH exposure, Actin mRNA served as an internal control. B H9c2 cells were transfected with miR-344b-5p mimics, miR-344b-5p inhibitor, and corresponding scrambled control. Relative miR-344b-5p expression was measure by qRT-PCR. C, D H9c2 cells were transfected with miR-344b-5p mimics or mimics NC and were treated with IH for six cycles. EdU staining assay was performed to evaluate the effect of miR-344b-5p on the proliferation of H9c2 cells, scale bar 50 µm. E H9c2 cells were transfected with miR-344b-5p mimics or mimics NC and were treated with IH for six cycles. Hoechst 33342/PI staining was performed to evaluate the effect of miR-344b-5p on the apoptosis of H9c2 cells, scale bar 50 µm. F H9c2 cells were transfected with miR-344b-5p mimics or mimics NC and were treated with IH for six cycles. Caspase-3 immunofluorescence was measured to show the apoptosis of H9c2 cells, scale bar 25 µm. G, H Expression levels of apoptosis-related proteins (Bcl-2, Bax and Cleaved caspase-3/Caspase-3) by western blot. *P < 0.05, **P < 0.001, ***P < 0.0001, ****P < 0.00001. Data were shown as mean ± SD based on three independent experiments.