Table 2 List of material used for immunostainings, in situ hybridization, and qRT-PCR experiments.
Antibodies | Catalog number | Manufacturer |
|---|---|---|
Immunostainings | ||
Goat anti-Trop2 | AF650 | R&D systems |
Mouse anti-Beta catenin | 610154 | BD transduction laboratories |
Rabbit anti-Olfactomedin 4 | #14369 | cell signaling tech |
TUNEL assay | 12156792910 | Roche |
DAPI | D9542 | Sigma-Aldrich |
Gene | Catalog number | Manufacturer |
RNAscope probes | ||
m Areg | 430501 | Advanced cell diagnostics |
m Axin 2 | 400331 | Advanced cell diagnostics |
m Hmox1 | 498811 | Advanced cell diagnostics |
m Lgr5 | 312171 | Advanced cell diagnostics |
m Pparg | 513371 | Advanced cell diagnostics |
m Pycard | 439581 | Advanced cell diagnostics |
Gene | Forward primer | Reverse primer |
qPCR primers | ||
Aqp1 | AGCTGGTACTGTGCGTTCTG | GTAGTCAATCGCCAGCAGGT |
Aqp3 | TGCCTTGCGCTAGCTACTTT | GAAGCATCTCCCCACAACGA |
Aqp4 | TGCCCGTAATCTGACTCCCA | AATGTCCACACTTACCCCACC |
Aqp5 | ATTGGCTTGTCGTCGGTCACACT | CCAGAAGACCCAGTGAGAGG |
Areg | GCGAATGCAGATACATCGAGAA | CGCTGTGAGTCTTCATGGATTTT |
Atf3 | GTCACCAAGTCTGAGGCGG | GTTTCGACACTTGGCAGCAG |
Axin2 | TGACTCTCCTTCCAGATCCCA | TGCCCACACTAGGCTGACA |
Casp1 | GACTGGGACCCTCAAGTTTTGCCC | CACCACCCTTCAGGATGGCCT |
Ccnd1 | CCTGCTACCGCACAACGCAC | GCCTGGCGCAGGCTTGACTC |
Cdkn1a | ATCCTGCAAGAGGCCTGGAGAGG | CACGAGGTCGGACGCCTATGGA |
Chga | TCCCCACTGCAGCATCCAGTTC | CCTTCAGACGGCAGAGCTTCGG |
Ddx60 | AGTGATGAGCCTTTGTTGAGGA | TCGTTCATGATGGCATCTCCC |
Egr1 | GAGCACCTGACCACAGAGTC | GCGGCCAGTATAGGTGATGG |
Hmox1 | CATAGCCCGGAGCCTGAATC | CAAATCCTGGGGCATGCTGT |
Hopx | GTGCCTGCGATCTTGGTGGCT | GCCTGACCTTACGTCTGTCCCG |
Lgr5 | CCTACTCGAAGACTTACCCAGT | GCATTGGGGTGAATGATAGCA |
Ly6A | GAAAGAGCTCAGGGACTGGAGTGTT | TTAGGAGGGCAGATGGGTAAGCAA |
Lyz1 | GAGACCGAAGCACCGACTATG | CGGTTTTGACATTGTGTTCGC |
Muc2 | ATGCCCACCTCCTCAAAGAC | GTAGTTTCCGTTGGAACAGTGAA |
Nupr1 | AATACCAACCGCCCTAGCC | TGTGGTCTGGCCTTATCTCC |
Olfm4 | CAGCTGCCTGGTTGCCTCCG | GGCAGGTCCCATGGCTGTCC |
Ptgs2 | CTGACCCCCAAGGCTCAAAT | TTTAAGTCCACTCCATGGCCC |
Pycard | GAGCAGCTGCAAACGACTAA | CTGGTCCACAAAGTGTCCTGT |
Rpl13 | CCCGTGGCGATTGTGAA | TCATTGTCCTTCTGTGCAGGTT |
Sis | TTCAAGAAATCACAACATTCAATTTACTAG | CTAAAACTTTCTTTGACATTTGAGCAA |
Slc7a11 | AGCTAACTGACTGCCCCTGG | ACTCAGAGGTGTGTTTCAGCC |
Trop2 | AGAACGCGTCGCAGAAGGGC | CGGCGGCCCATGAACAGTGA |
Wwtr1 | CGGTTCCGGGGATGTAAGAG | GAACTGACGAGCTGGAACCC |
Ywhaz | TGCAACGATCTACTGTCTCTTTTG | CGGTAGTAGTCACCCTTCATTTTCA |
