Fig. 3: The expression of IGF2BP3 is regulated by MYC, which acts as a transcription factor. | Cell Death Discovery

Fig. 3: The expression of IGF2BP3 is regulated by MYC, which acts as a transcription factor.

From: MYC-activated RNA N6-methyladenosine reader IGF2BP3 promotes cell proliferation and metastasis in nasopharyngeal carcinoma

Fig. 3

a, b The genetic alterations of IGF2BP3 in TCGA data predicted by cBioPortal. c Diagram of transcription factors MYC binds to the promoter region of IGF2BP3. d Luciferase reporter assay verified the direct target binding of MYC and IGF2BP3. e CHIP assay was performed to measure the connection between MYC and IGF2BP3 in NPC cells. The enrichment of ChIP is normalized by input DNA via qRT-PCR. f–h qRT-PCR and western blot results of MYC and IGF2BP3 mRNA and protein expression in 5–8 F and CNE2 cells after MYC knockdown. Data was shown as the mean ± standard deviation of three independent experiments. *P < 0.05, **P < 0.01.

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