Fig. 4: IGF2BP3 regulates the mRNA stability of KPNA2 by reading its m6A modification site. | Cell Death Discovery

Fig. 4: IGF2BP3 regulates the mRNA stability of KPNA2 by reading its m6A modification site.

From: MYC-activated RNA N6-methyladenosine reader IGF2BP3 promotes cell proliferation and metastasis in nasopharyngeal carcinoma

Fig. 4

a Correlation between IGF2BP3 and KPNA2 in GSE13597. b Correlation of H-score between IGF2BP3 and KPNA2 in immunohistochemistry. c, d IHC staining of IGF2BP3 and KPNA2 in IGF2BP3 low specimens (c) and IGF2BP3 high specimens (d). e RIP assay analysed KPNA2 enrichment in IGF2BP3 in NPC cells. The enrichment of RIP was normalized by input RNA via qRT-PCR. f, g qRT-PCR and western blot results of IGF2BP3 and KPNA2 mRNA and protein expression in 5–8 F and CNE2 cells after IGF2BP3 knockdown. h Treated transfected NPC cells with actinomycin D for indicated times, and the mRNA levels of KPNA2 were measured by qRT‐PCR. i Possible sequence and structure of m6A sites on KPNA2 transcripts predicted by SRAMP. j Motif display and metagene analysis of m6A sites on KPNA2 transcripts via RMBase V2.0. k MeRIP-qPCR analysis of KPNA2 mRNA. l MeRIP-qPCR analysis of KPNA2 mRNA after IGF2BP3 knockdown, compared to the negative control. Data was shown as the mean ± standard deviation of three independent experiments. **P < 0.01.

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