Fig. 2: PHLPP1 depletion promotes β-cell survival in the obese db/db mouse model of type 2 diabetes.
A Insulin-positive area and B β-cell mass analysis given as a mean percentage of the entire pancreatic section area from 10 sections/mouse throughout the whole pancreas, C the respective β-cell size analysis from 100 randomly chosen β-cells from each group and D representative insulin staining of the pancreas. E–H Quantitative analyses and representative images of E, F double-staining for TUNEL and G, H triple-staining for Ki67 both expressed as a percentage of insulin-positive β-cells (an average of 3827 and 3965 β-cells/ mouse were counted for TUNEL and Ki67 analyses, respectively for each group). Quantitative analyses (I, K) and representative images (J) of the percentage of glucagon-positive α-cells (red) and insulin-positive β-cells (green) together with quantitative analyses of insulin and glucagon colocalization (L; an average of 1455 β-cells and 529 α-cells/mouse were counted for each group). M, N Representative images for double-staining of M nuclear PDX1 and N GLUT2 expression. Data show means from all pooled analyses for each mouse ± SEM. *p < 0.05 db/db-PHLPP1−/− vs. db/db-PHLPP1+/− controls; all by Student’s t-tests. Scale bars depict 50 µm in D and 10 µm in F, H, J, M, N.
