Fig. 6: Ectopic expression of miR-578 inhibited proliferation, pro-inflammatory cytokine secretion, and ECM degradation in NP cells.
From: Hsa_circ_0134111 promotes intervertebral disc degeneration via sponging miR-578
A miR-578 overexpression inhibited NP cell proliferation as shown by the CCK-8 assay. B The expression of cyclin D1 was analyzed by using qRT-PCR and GAPDH was used as the internal control. C–F Overexpression of miR-578 suppressed C IL-6, D IL-8, E MMP-9, and F ADAMTS-5 expression as shown by qRT-PCR. G, H The expression of type II collagen and aggrecan was determined by qRT-PCR assay. Ectopic expression of circ_0134111 decreased G type II collagen and H aggrecan expression in NP cells. J The protein expression of MMP-9 and ADAMTS-5 was measured by western blots. *p < 0.05, **p < 0.01, and ***p < 0.001.
