Fig. 1: Oxidative stress induces caspase- and necroptosis-independent cell death in melanocytes. | Cell Death Discovery

Fig. 1: Oxidative stress induces caspase- and necroptosis-independent cell death in melanocytes.

From: Oxeiptosis: a novel pathway of melanocytes death in response to oxidative stress in vitiligo

Fig. 1: Oxidative stress induces caspase- and necroptosis-independent cell death in melanocytes.

A The safety concentration of Z-VAD that does not affect cell viability in PIG1 cells was determined by CCK-8 assay. B The safety concentration of Nec-1 that does not affect cell viability in PIG1 cells was determined by CCK-8 assay. The cell viability of PIG1 cells treated by H2O2 or pretreated with Z-VAD and Nec-1 prior to H2O2 disposition is determined by CCK8 assay (C) and Resazurin-conversion assay (D). E The activity of released LDH in PIG1 cells treated by H2O2 or pretreated with Z-VAD and Nec-1 prior to H2O2 disposition was determined by LDH to release assay. F Annexin V-FITC/PI apoptosis assay of PIG1 cells pretreated with Z-VAD and Nec-1, followed by treatment with H2O2. *P < 0.05, **P < 0.01, ***P < 0.001, ns: not significant.

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