Fig. 7: Knockdown of hsa_circ_0001394 suppresses HCC progression in vivo. | Cell Death Discovery

Fig. 7: Knockdown of hsa_circ_0001394 suppresses HCC progression in vivo.

From: The circular RNA hsa_circ_0001394 promotes hepatocellular carcinoma progression by targeting the miR-527/UBE2A axis

Fig. 7

A Five pairs of nude mice were subcutaneously injected with Huh7 cells transfected with sh-NC or sh-circRNA plasmids. After 30 days, HCC tumors were dissected and photographed. B Tumor weight was calculated on the day mice were euthanized. The tumor weight of hsa_circ_0001394-deficient group was markedly lower than that of the control group. C Tumor volumes were recorded every 5 days starting on the day when mice were inoculated with Huh7 cells transfected with sh-NC or sh-circRNA. Hsa_circ_0001394 knockdown decreased tumor volumes relative to the control group. D Hsa_circ_0001394 was downregulated in the hsa_circ_0001394-deficient group relative to the control group according to qRT-PCR. E MiR-527 was upregulated in the sh-circRNA group compared to the control group according to qRT-PCR. F UBE2A was significantly expressed at lower levels in the hsa_circ_0001394 knockdown group than in the control group as determined by qRT-PCR. G The protein levels of UBE2A were compared between the sh-NC nude mouse models and sh-circRNA models using western blot analysis. Hsa_circ_0001394 silencing reduced the protein levels of UBE2A. H Representative images of hematoxylin–eosin (HE) staining of sections of xenograft tumors formed by Huh7 cells. Ki-67 staining of tumors was determined by immunohistochemical analysis. All values are presented as mean ± SD from three independent experiments with similar results. (*p < 0.05, **p < 0.01, ***p < 0.001).

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