Fig. 2: CBD and cannabis extracts enhance expression of miR-200c-3p and let-7a-5p through AKT pathway.
From: New AKT-dependent mechanisms of anti-COVID-19 action of high-CBD Cannabis sativa extracts
A, B qRT-PCR analysis of miR-200b-3p, miR-200c-3p, let-7a-5p and let-7b-5p in WI-38 cells treated with either CBD or the selected extracts. C Diagram of luciferase reporter bearing either wild-type or mutant ACE2 3′UTR or TMPRSS2 3′UTR; the mutated deoxy nucleotides are underlined. D Luciferase assay was performed as described in the Methods section. E Western blot analysis of pAKT1/2/3 and AKT1/2/3 in WI-38 cells treated with MK2206 for 2 h prior to the addition of CBD or the selected extracts. F qRT-PCR analysis of miR-200c-3p or let-7a-5p in WI-38 cells treated with MK2206 for 2 h prior to the addition of CBD or the selected extracts. * indicates p < 0.05.
