Fig. 5: The selected cannabis extracts regulate the expression of proinflammatory cytokines triggered by TNFα/IFNγ and affect signaling pathways.
From: New AKT-dependent mechanisms of anti-COVID-19 action of high-CBD Cannabis sativa extracts
A–C Western blot analysis of the indicated proteins in WI-38 cells treated with either TNFα/IFNγ alone or in combination with the indicated extracts; relative densitometry was measured using ImageJ. D qRT-PCR analysis of IL-6, IL-8, and COX2 in WI-38 cells treated with either TNFα/IFNγ alone or in combination with the indicated extracts. E, F qRT-PCR analysis of IL-6 and IL-8 in WI-38 cells treated with either TNFα/IFNγ alone or in combination with extract #1 or #5 for the indicated times. G Western blot analysis of pAKT/1/2/3 and AKT1/2/3 in WI-38 cells treated with MK2206 for 2 h prior to the addition of either TNFα/IFNγ or in combination with the indicated extracts. H qRT-PCR analysis of IL-6 and IL-8 in WI-38 cells treated with MK2206 for 2 h prior to the addition of either TNFα/IFNγ or in combination with the indicated extracts. * indicates p < 0.05.
