Fig. 3: c-Myb overexpression decreases cochlear HC apoptosis induced by cisplatin.
From: c-Myb protects cochlear hair cells from cisplatin-induced damage via the PI3K/Akt signaling pathway
A Representative images of TUNEL staining showing that apoptotic cochlear HCs exhibited both TUNEL (red) and myosin7a (green) fluorescence after cisplatin treatment. Scale bar (low magnification) = 20 μm. Scale bar (high magnification) = 5 μm. B Statistical analysis verified that the numbers of TUNEL/myosin 7a double-positive IHCs and OHCs in the cisplatin + AAV-c-Myb group were reduced significantly compared to the cisplatin + AAV-ie group. C Representative images of cleaved-caspase3 staining showing that cleaved-caspase3 fluorescence (red) was present in the apoptotic HCs (myosin 7a labeling, green) after cisplatin exposure. Scale bar (low magnification) = 20 μm. Scale bar (high magnification) = 5 μm. D Cell counting and statistical analysis confirmed that the number of cleaved-caspase3/myosin 7a double-positive IHCs and OHCs in the cochlear basal turns was increased significantly after cisplatin treatment compared to the vehicle control group, whereas the number of double-positive cells was decreased significantly in the cisplatin + AAV-c-Myb group compared with the cisplatin + AAV-ie group. **p < 0.01, ***p < 0.001. n = 6 for each group.
