Fig. 3: MEG3 targeted EZH2 to ubiquitination-mediated degradation. | Cell Death Discovery

Fig. 3: MEG3 targeted EZH2 to ubiquitination-mediated degradation.

From: LncRNA MEG3 up-regulates SIRT6 by ubiquitinating EZH2 and alleviates nonalcoholic fatty liver disease

Fig. 3: MEG3 targeted EZH2 to ubiquitination-mediated degradation.

A EZH2 expression was examined by Western blot in MEG3-expressing vs. vector and in si-MEG3-transfected vs. si-NC-transfected hepatocytes. B Interaction between MEG3 and EZH2 was examined by RIP assay. IgG was used as the negative control. C Indicated hepatocytes were treated with MG132 for 0, 4, and 8 h, respectively. EZH2 level was detected by Western blot. D Indicated hepatocytes were treated with cycloheximide for 0, 2, 4, and 8 h, respectively. EZH2 level was detected by Western blot. E Ubiquitinated EZH2 was detected by co-immunoprecipitation using either anti-EZH2 or IgG. F The levels of phosphorylated EZH2 at T345 and T487 were examined by Western blot in indicated hepatocytes. *P < 0.05, ***P < 0.001.

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