Fig. 2: High-dose-DHT suppresses EnzR PCa cell growth via induction of autophagic cell death. | Cell Death Discovery

Fig. 2: High-dose-DHT suppresses EnzR PCa cell growth via induction of autophagic cell death.

From: High-dose-androgen-induced autophagic cell death to suppress the Enzalutamide-resistant prostate cancer growth via altering the circRNA-BCL2/miRNA-198/AMBRA1 signaling

Fig. 2

A BCL2 mRNA expression in EnzR1-C4-2 and EnzR4-C4-2B cell lines treated with/without (w/wo) 50 nM DHT. B BCL2 protein expression in EnzR1-C4-2 and EnzR4-C4-2B cell lines treated w/wo 50 nM DHT. C, D Western blot assays were performed on EnzR1-C4-2 (C) and EnzR4-C4-2B (D) cell lines to show that caspase-dependent apoptosis marker is decreased with 50 nM DHT treatment. E, F Western Blot was performed on EnzR1-C4-2 and EnzR4-C4-2B cell lines to show that autophagy markers LC3 (LC3-I and LC3-II) are increased (E) and p62 is decreased (F) with 50 nM DHT treatment. G, H GFP-LC3 plasmid transfection was performed using Lipofectamine 3000 transfection reagent on EnzR1-C4-2 (G) and EnzR4-C4-2B (H) cells to show that GFP-LC3 puncta positive cells increased with 50 nM DHT treatment. The fluorescence images were captured by microscopy, scale bar = 40 µm. Data are presented as means ± SD. *p < 0.05.

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