Fig. 5: Adoptive transfer of OPN−/− BMDM reduces tissue injury in mice induced by subsequent PR8 infection. | Cell Death Discovery

Fig. 5: Adoptive transfer of OPN−/− BMDM reduces tissue injury in mice induced by subsequent PR8 infection.

From: Osteopontin aggravates acute lung injury in influenza virus infection by promoting macrophages necroptosis

Fig. 5

A–E WT or OPN−/− BMDMs were intranasally transferred to WT recipient mice. One day after transfer, mice were infected with PR8 (1.5 × 103 PFU/animal) and lung tissues were collected 24 h after infection. A Diagrammatic representation of adoptive transfer. B Serial lung sections of representative lungs stained with hematoxylin and eosin, scale bar = 100 μm. C Inflammatory factor (IL-6, MCP-1, and IFN-γ) levels in lung homogenates were measured using qPCR. D Representative images of immunofluorescence staining for P-MLKL in the lung. E Lung homogenates were collected and subjected to western blot analysis. Each lane corresponds to an individual mouse. Lane-loading differences were normalized by levels of ACTIN. Data was analyzed by one-way ANOVA and expressed as mean ± SEM. n = 3-4/group. *p < 0.05; ****p < 0.0001; ns denote no statistical significance.

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