Fig. 5: Abnormal p53 expression affects trophoblast cell survival. | Cell Death Discovery

Fig. 5: Abnormal p53 expression affects trophoblast cell survival.

From: The possible involvement of circRNA DMNT1/p53/JAK/STAT in gestational diabetes mellitus and preeclampsia

Fig. 5

A HTR-8/SVneo cells were transiently transfected with p53 pcDNA3.1 (OE-p53) or siRNA (si-p53) for 48 h. qRT-PCR analysis upregulated expression of p53. B CCK-8 assays were implemented to detect the viability of OE-p53 or si-p53-treated HTR-8/SVneo cells. C BrdU assays were implemented to detect the proliferation of OE-p53- or si-p53-treated HTR-8/SVneo cells. D The apoptotic rates of HTR-8/SVne cells transfected with OE-p53 or si-p53 were measured by flow cytometry. E The cell cycle of HTR-8/SVne cells transfected with OE-p53 or si-p53 was measured by flow cytometry. F Wound scratch assay was performed to detect the role of OE-p53- or si-p53-treated HTR-8/SVne cell migration in vitro. G Transwell assays were performed to detect the invasion of HTR-8/SVne cells transfected with OE-p53 or si-p53. **P < 0.001 compared with si-NC. ##P < 0.001 compared with OE-NC. N = 3, repetition = 3.

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