Fig. 7: IL-6 partially abolishes the inhibitory effect of Stattic in PCCs via STAT3. | Cell Death Discovery

Fig. 7: IL-6 partially abolishes the inhibitory effect of Stattic in PCCs via STAT3.

From: Inhibition of STAT3Y705 phosphorylation by Stattic suppresses proliferation and induces mitochondrial-dependent apoptosis in pancreatic cancer cells

Fig. 7

PCCs were treated with 10 μM of Stattic with or without IL-6 for 24 h. A Expression and phosphorylation of STAT3 and JAK2 in the indicated groups. B Immunocytochemical staining of p-STAT3 in Stattic-treated PANC-1 and BxPc-3 cells with or without IL-6. Bar = 50 μm. C Colony formation assay revealing the proliferation of Stattic-treated PANC-1 and BxPc-3 cells with or without IL-6. D Expression of Ki67 in Stattic-treated cells with or without IL-6. E Immunocytochemical staining of Ki67 in Stattic-treated PANC-1 and BxPc-3 cells with or without IL-6. Bar = 50 μm. F Expression of c-Myc and p53 activity in cells treated with or without IL-6. G Expression and phosphorylation of cyclin D1 in Stattic-treated PANC-1 and BxPc-3 cells with or without IL-6. H Western blot analysis showing the expression of Bcl-2, Bax, FL-Casp3, and CL-Casp3 in Stattic-treated PANC-1 and BxPc-3 cells with or without IL-6. *P < 0.05.

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