Fig. 8: Lentivirus-mediated overexpression of STAT3 partially reverses the anti-tumor effect of Stattic. | Cell Death Discovery

Fig. 8: Lentivirus-mediated overexpression of STAT3 partially reverses the anti-tumor effect of Stattic.

From: Inhibition of STAT3Y705 phosphorylation by Stattic suppresses proliferation and induces mitochondrial-dependent apoptosis in pancreatic cancer cells

Fig. 8

A Expression of STAT3 without Stattic-treated PANC-1 cells after transfection with lentivirus. B Immunocytochemical staining of p-STAT3 in Stattic-treated PANC-1 cells after transfection with lentivirus. Bar = 50 μm. C Colony formation assay revealing the proliferation of Stattic-treated PANC-1 cells after transfection with lentivirus. D The expression of Ki67 in Stattic-treated PANC-1 cells after transfection with lentivirus. E Immunocytochemical staining of Ki67 in Stattic-treated PANC-1 cells after transfection with lentivirus. Bar = 50 μm. F Expression and phosphorylation of p53 in Stattic-treated PANC-1 cells after transfection with lentivirus. G Expression and phosphorylation of cyclin D1 in Stattic-treated PANC-1 cells after transfection with lentivirus. H Proteins levels of Bcl-2, CL-Casp3, Bax, and FL-Casp3 in PANC-1 cells subjected to the indicated treatments. **P < 0.01.

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