Fig. 5: Autophagy is reduced in CSCs exposed to high-glucose concentration. | Cell Death Discovery

Fig. 5: Autophagy is reduced in CSCs exposed to high-glucose concentration.

From: The impact of autophagy modulation on phenotype and survival of cardiac stromal cells under metabolic stress

Fig. 5: Autophagy is reduced in CSCs exposed to high-glucose concentration.

Representative WB panels (A) and relative densitometric quantification (B) of LC3-II (n=8), ATG5, and ATG7 protein expression after exposure for 24 h to 5 mM or 50 mM glucose (n=3). C ATG7 mRNA expression levels after 24 h of culture in 5 mM or 50 mM glucose (n=5). Representative WB panels (D) and relative densitometric quantification (E) of LC3-II expression profile after 24 h of exposure to 50 mM glucose, with or without treatment for 2 hours with 100 nM bafilomycin A1 (BAF) (n=4). LC3-I and II panel is displayed twice with different exposure times, for the optimized detection of the two bands (higher exposure, top panel; lower exposure, lower panel). F Representative fluorescence microscopy images of Ad-mRFP-GFP-LC3 transduced cells, exposed for 24 h to 50 mM glucose or 5 mM glucose as control. G Corresponding quantification of the number of mRFP+/GFP+ autophagosomes per cell surface area in 50 mM glucose or 5 mM control (n=3). Scale bars=20 µm. *P<0.05, **P<0.01.

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