Fig. 2: METTL3 silencing suppressed malignant proliferation of OS cells. | Cell Death Discovery

Fig. 2: METTL3 silencing suppressed malignant proliferation of OS cells.

From: METTL3 stabilizes HDAC5 mRNA in an m6A-dependent manner to facilitate malignant proliferation of osteosarcoma cells

Fig. 2

Three Three METTL3 siRNAs (si-METTL3#1, si-METTL3#2, and si-METTL3#3) were transfected into U2OS cells with relatively high METTL3 expression, with si-NC as negative control. pcDNA 3.1 METTL3 (pc-METTL3) was transfected into HOS cells with relatively low METTL3 expression, with pc-NC as negative control. After 48 h, A, B METTL3 expression in OS cells was detected using RT-qPCR and western blot. Two siRNAs with high transfection efficiency (si-METTL3#1 and si-METTL3#2) were selected for subsequent experimentation. B–D The proliferation of OS cells was measured using CCK-8 assay (B), colony formation assay (14 days) (C), and EdU staining (D). The cell experiment was repeated three times independently. Data are presented as mean ± standard deviation. Data in panels A, B/D–E were analyzed using one-way ANOVA, and data in panel C were analyzed using two-way ANOVA, followed by Tukey’s multiple comparisons test, *p < 0.05, **p < 0.01.

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