Fig. 6: miR-142-5p targeted ARMC8. | Cell Death Discovery

Fig. 6: miR-142-5p targeted ARMC8.

From: METTL3 stabilizes HDAC5 mRNA in an m6A-dependent manner to facilitate malignant proliferation of osteosarcoma cells

Fig. 6

A The target genes of miR-142-5p were predicted through Starbase, miRWalk, Targetscan, and miRDB databases. B The binding site of miR-142-5p and ARMC8 in the Starbase database. C, D The binding of miR-142-5p and ARMC8 in OS cells was verified using dual-luciferase assay and RNA pull-down. E ARMC8 mRNA level in 50 pairs of OS tissues and adjacent tissues was determined using RT-qPCR. F ARMC8 mRNA level in human OS cell lines (U2OS, HOS, SAOS2) and normal osteoblasts (hFOB1.1) was determined using RT-qPCR. G The correlation between ARMC8, miR-142-5p, HDAC5, and METTL3 in OS tissues was analyzed using Pearson correlation analysis. H ARMC8 mRNA level in OS cells after intervention with METTL3 and HDAC5 expression was measured using RT-qPCR. N = 50. The cell experiment was repeated three times independently. Data in panels C-D/F-H are presented as mean ± standard deviation. Data in panel E were analyzed using paired t-test. Data in panels F/H were analyzed using one-way ANOVA, and data in panels C, D were analyzed using two-way ANOVA, followed by Tukey’s multiple comparisons test, *p < 0.05, **p < 0.01.

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