Fig. 5: HORMAD1 activates the Wnt/β-catenin pathway in lung cancer cells. | Cell Death Discovery

Fig. 5: HORMAD1 activates the Wnt/β-catenin pathway in lung cancer cells.

From: The cancer/testis antigen HORMAD1 mediates epithelial–mesenchymal transition to promote tumor growth and metastasis by activating the Wnt/β-catenin signaling pathway in lung cancer

Fig. 5

Western blotting and quantitative analyses of total, cytosolic, and nuclear β-catenin in H157 cells with or without HORMAD1 expression (A) and in NC and HORMAD1 KO H650 cells (B). α-tubulin and Lamin B1 were used as the loading controls for the cytoplasmic and nuclear fractions, respectively. Immunofluorescence staining analyses of the subcellular β-catenin localization in H157 cells with or without HORMAD1 expression (C) and in NC and HORMAD1 KO H650 cells (D); scale bars are 100 μm. TOP-Flash/FOP-Flash luciferase reporter assay of β-catenin transcriptional activity in H157 cells with or without HORMAD1 expression (E) and in NC and HORMAD1 KO H650 cells (F). The relative mRNA levels of Axin-2, Cyclin-D1, c-Myc, MMP-7, and MMP-9 in H157 cells with or without HORMAD1 (G) and NC and HORMAD1 KO H650 cells (H) were analyzed by quantitative real-time PCR. Western blotting and quantitative analyses of E-cadherin, N-cadherin, Vimentin, ZEB-1, and β-catenin protein levels in H157 cells with or without HORMAD1 expression (I) and in NC and HORMAD1 KO H650 cells (J) after transfection with β-catenin siRNA. All histograms representing indicated the results of three independent experiments. Mean ± SEM from three independent experiments are shown. **p < 0.01; *** p < 0.001; ns, not significant.

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