Fig. 4: GRP78 inhibition enhances TAK-243-induced ER stress and UPR in GBM cells.
From: GRP78 blockade overcomes intrinsic resistance to UBA1 inhibitor TAK-243 in glioblastoma
A Representative western blot analysis showing the effects of TAK-243 combined with HA15 on the ER stress and UPR in U87 and LN229 cells. B U87 and LN229 cells were treated with TAK-243 (100 nM) and/or HA15 (10 μΜ) for 24 h. Caspase 3/7 activity were assessed by Caspase-Glo 3/7 activity assay. C U87 cells were treated with indicated inhibitors for 12 h, and then cell viability was assessed by CCK-8 assay. D The protein levels of cleaved PARP, Bax and Bcl-2 were assessed by immunoblotting in U87 and LN229 cells treated by TAK-243 and/or HA15. All the data were presented as means ± SD. *P < 0.05, ***P < 0.001.
