Fig. 6: NF-κB agonist Asatone rescues the function of promoting enzalutamide resistance in ABCC5-knockdown cells. | Cell Death Discovery

Fig. 6: NF-κB agonist Asatone rescues the function of promoting enzalutamide resistance in ABCC5-knockdown cells.

From: Non-drug efflux function of ABCC5 promotes enzalutamide resistance in castration-resistant prostate cancer via upregulation of P65/AR-V7

Fig. 6: NF-κB agonist Asatone rescues the function of promoting enzalutamide resistance in ABCC5-knockdown cells.The alternative text for this image may have been generated using AI.

A Representative images of confocal microscopy immunofluorescence staining for ABCC5 and p-P65 co-localization in 22RV1WT and 22RV1Enza cells. Scale bars, 20 μm. The levels of ABCC5, AR-V7, phosphorylated IKBα, and phosphorylated P65 were detected by Western blotting in ABCC5-knockdown and control C4-2BEnza (B) and 22RV1Enza (C) cells combined with or without Asatone (5 µM) in enzalutamide treatment. D ABCC5-knockdown and control C4-2BEnza and 22RV1Enza cells with or without Asatone in enzalutamide treatment for colony formation assays construction. E Relative mRNA expression of AR and AR-V7 target genes (PSA, and SREBF1) were analyzed by qRT-PCR under the condition of C4-2B shNC and shABCC5 cells treated with or without 5 μM Asatone accompanied by 30 μM enzalutamide for 48 h. *P < 0.05, ***P < 0.001, n.s., not significant.

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