Fig. 1: Cbl-b and c-Cbl deficiency in DCs results to liver cirrhosis and untimely death of mice. | Cell Death Discovery

Fig. 1: Cbl-b and c-Cbl deficiency in DCs results to liver cirrhosis and untimely death of mice.

From: Ablation of Cbl-b and c-Cbl in dendritic cells causes spontaneous liver cirrhosis via altering multiple properties of CD103+ cDC1s

Fig. 1

A Genomic DNA was extracted and PCR was performed to detect levels of Cbl-b and c-Cbl in mice. B Bone marrow cells were cultured with 10 ng/ml GM-CSF for 7 days followed by magnetic bead isolation of CD11c-positive cells. Protein levels of Cbl-b and c-Cbl in CD11c+ cells were quantified by Western blot. C Survival curves of four groups of mice (n = 17 per group). D Livers of mice of all four genotypes were dissected for morphologic examination (n = 3 per group). E Livers were removed and fixed with 10% formalin. Paraffin sections were stained with hematoxylin and eosin (n = 3 per group); scale bar, 50 μm. F Livers were dissected and fixed with 10% formalin. Paraffin sections were stained with Sirius red for 1 h (n = 3 per group); scale bar, 100 μm. ****p < 0.0001 by log-rank tests. p < 0.05 was considered statistically significant.

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