Fig. 1: Generation and characterization of WT and AD hiPSCs-derived brain organoids.
From: Zika virus infection accelerates Alzheimer’s disease phenotypes in brain organoids
A A schematic procedure for generating brain organoids from hiPSCs. Representative bright-field images at different stages. B Clearing of whole organoids immunostained for neural progenitor marker SOX2 and the neuronal marker TUJ1 in WT and AD brain organoids at day 30. Scale bars, 500 µm. C Immunostaining of the neural progenitor marker SOX2 and the neuronal marker TUJ1 in WT and AD organoids at day 60. Scale bars, 100 µm. D Immunostaining of the cortical deep-layer marker CTIP2 and the intermediate progenitor marker TBR2 in WT and AD organoids at day 60. Scale bars, 100 µm. E Immunostaining of the neural progenitor marker SOX2 and the cortical upper layer marker SATB2 in WT and AD organoids at day 60. Scale bars, 100 µm. F Immunostaining of the proliferation marker Ki67 and the neuronal marker MAP2 in WT and AD organoids at day 60. Scale bars, 100 µm. G Immunostaining of the astrocyte marker GFAP in WT and AD organoids at day 60. Scale bars, 100 µm. Every experiment was performed in triplicate.
