Fig. 7: IL-37D inhibits sST2 production in IL-1R8-dependent manner.

a–d ADSCs (1 × 10⁵/mL) from inguinal WAT of WT and IL-37Dtg mice were stimulated by TNF-α (10 ng/mL) for 24 h. The mRNA (a) and secretion (b) levels of sST2 were detected by qPCR and ELISA. The protein level of p-P65 was detected by western blot (c), the grayscale values are shown (d). e–h ADSCs from inguinal WAT of WT mice were treated with IL-37D protein (1 ng/mL) in the presence or absence of TNF-α (10 ng/mL) for 24 h. The mRNA (e) and secretion (f) levels of sST2 were detected by qPCR and ELISA. The protein level of p-P65 was detected by western blot (g) and the grayscale values are shown (h). IL-1R8 was silenced in ADSCs from IL-37Dtg mice, the mRNA levels of IL-1R8 (i) and sST2 (j) were detected by qPCR, the secretion level of sST2 was detected by ELISA (k), and the protein level of p-P65 was detected by western blot (l) and the grayscale values are shown (m). Data in a–m are representative of three independent experiments. Data represent mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 determined by two-way ANOVA (a, b, d–f, h, j, k, m) or student’s t test (i).