Fig. 4: CircRPAP2 and SRSF1 are involved in the alternative splicing of PTK2.
From: CircRPAP2 regulates the alternative splicing of PTK2 by binding to SRSF1 in breast cancer
A and B Expression levels of PTK2 mRNA and protein were detected by qRT-PCR and western blotting in BC cells when circRPAP2 was depleted or overexpressed. C and D Expression levels of PTK2 mRNA and protein were detected by qRT-PCR and western blotting in BC cells when SRSF1 was knocked down or overexpressed. E Expression level of PTK2 pre-mRNA was detected by qRT-PCR in BC cells when circRPAP2 was depleted or overexpressed. F Expression level of PTK2 pre-mRNA were detected by qRT-PCR in BC cells when SRSF1 was knocked down or overexpressed. G PTK2 mRNA is upregulated in BC tissues (n = 102). H PTK2 mRNA is upregulated in BC cell lines. I PTK2 pre-mRNA is downregulated in BC tissues (n = 102). J PTK2 pre-mRNA is downregulated in BC cell lines. K Negative correlation between the expression of circRPAP2 and PTK2 mRNA in BC tissue samples. (n = 102, Y = −1.503*X + 2.810, R2 = 0.662, P < 0.001). L Positive correlation between the expression of circRPAP2 and PTK2 pre-mRNA in BC tissue samples. (n = 102, Y = 1.339*X – 3.063, R2 = 0.605, P < 0.001). (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001).
