Fig. 6: Rescue assay of the circRPAP2/SRSF1/PTK2 axis.
From: CircRPAP2 regulates the alternative splicing of PTK2 by binding to SRSF1 in breast cancer
A–C CircRPAP2 overexpression-induced cell proliferation and migration can be rescued by overexpression of SRSF1 as determined using by colony formation (A), MTT (B), and Transwell migration assays (C). D–I CircRPAP2 overexpression-mediated reduction in PTK2 mRNA and protein expression and increase in PTK2 pre-mRNA could be recovered by overexpressing SRSF1 in BC cells as determined by qRT-PCR and western blotting. J CircRPAP2 overexpression-mediated reduction in PTK2 protein expression could not be recovered by mutant of SRSF1 binding site in BC cells as determined by western blotting. (*P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, ns: no significance).
