Fig. 4: FAM84B interacted with NPM1 in ESCC cells. | Cell Death Discovery

Fig. 4: FAM84B interacted with NPM1 in ESCC cells.

From: Elevated FAM84B promotes cell proliferation via interacting with NPM1 in esophageal squamous cell carcinoma

Fig. 4

A The co-location of endogenous FAM84B and NPM1 by immunofluorescence in KYSE150 cells. The first image of cells was stained with the first antibody of anti-FAM84B and the second antibody of Alexa FluorTM 594 goat anti-rabbit (red); the second image of cells was stained with the first antibody of anti-NPM1 and Alexa Fluor® 488 donkey anti-mouse (green); the third image nuclei of cells were stained with DAPI (blue); the last image was merged (yellow). B The interaction with FAM84B and NPM1 was detected in endogenous cells by CO-IP assay. C The interactions with FAM84B and NPM1 were detected in exogenous cells by CO-IP assay. D The interaction directly with FAM84B and NPM1 was detected in 293 T cells by GST-pull-down assay.

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