Fig. 5: NPM1 may be a downstream target of FAM84B in ESCC. | Cell Death Discovery

Fig. 5: NPM1 may be a downstream target of FAM84B in ESCC.

From: Elevated FAM84B promotes cell proliferation via interacting with NPM1 in esophageal squamous cell carcinoma

Fig. 5

A Schematic representation of full-length and deletion mutants of HA-tagged NPM1 protein. B The FLAG-FAM84B protein was incubated with full-length and deletion mutants of HA-tagged NPM1 protein using CO-IP assay. C The NPM1 was examined after transfecting pCMV-FLAG-FAM84B in gradient amount. D The NPM1 nuclear expression was detected in FAM84B over-expression cells. E Detected the efficiency of knockdown NPM1 in stably over-expression FAM84B cells by western blot. F Knock-down NPM1 inhibited the capability of cell proliferation in stably over-expression FAM84B cells. G Knock-down NPM1 inhibited the capability of colony formation in stably over-expression FAM84B cells. H Knock-down NPM1 inhibited cell cycle in stably over-expression FAM84B cells. Statistical analysis was performed using one-way ANOVA. ***P < 0.001.

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