Fig. 6: FAM84B-NPM1 might regulate cell cycle via suppressing the expression of CDKN2A.

A The interaction with FAM84B and CDKN2A were detected in cells by CO-IP assays. B The interaction with NPM1 and CDKN2A were detected in cells by CO-IP assays. C NPM1 over-expression decreased the CDKN2A expression in 150 and 450 cells. D Deteced the expression of cell cycle proteins by western blot in over-expression/knockdown FAM84B cells. E Immunohistochemical images showed the expression level of Ki-67, FAM84B, NPM1, CDK4, and CCND1 from mice injected with KYSE150 NC cells and FAM84B knockdown cells, Magnification, ×200. F Diagram showing how copy number amplification of FAM84B contributes to tumorigenesis of ESCC via regulation of cell cycle. G Schematic diagram of the molecular mechanism that FAM84B copy number amplification promoted cell proliferation in ESCC. Statistical analysis was performed using one-way ANOVA. ***P < 0.001.