Fig. 3: Effect of IRX5 knockdown on the adipogenic differentiation of hMSCs.
From: IRX5 promotes adipogenesis of hMSCs by repressing glycolysis
A, B IRX5 knockdown efficiency were verified by qRT-PCR (A) and western blot (B). B IRX5 and CEBP-α protein expression. C Oil red O staining analysis of lipid droplets at D16 after adipogenic differentiation. D qRT-PCR analysis of PPAR-γ, CEBP-α, CD36 and FABP4 at D8 after adipogenic differentiation. Scale bar, 500 μm. Results are shown as mean ± SD. n = 3. * Indicates a significant difference from the control group, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.
